John Bumpus

John Bumpus

Professor - Biochemistry, Environmental Chemistry, Mycology
Department of Chemistry
University of Northern Iowa
Cedar Falls, IA 50614-0423

Office: 285 McCollum Science Hall
Voice: 319-273-7220
E-mail: John.Bumpus@uni.edu

Degrees

  • B.S., 1971, State University of New York, Oswego, NY
  • M.A. 1975, State University of New York, Binghampton, NY
  • Ph.D., 1980, St. Louis University; St. Louis, MO.

Research Interests

My research focuses on harnessing the unique biodegradative abilities of the wood-rotting fungus Phanerochaete chrysosporium for a variety of applications including bioremediation of contaminated soil and water. Lignin and cellulose are structural components of wood that are responsible for its durability and suitability as a building material. One of the characteristics of wood that make it suitable for such purposes is that the Iignin component is relatively resistant to attack and decay by microorganisms. Furthermore, it is thought that lignin also protects cellulose from microbial decay.

Lignin, a non-repeating heteropolymer, is the second most abundant renewable carbon compound on earth. Only cellulose is more abundant. This, combined with the fact that only a relatively few microorganisms have the ability to degrade lignin, makes its turnover the rate-limiting step in the carbon cycle. Although lignin is clearly resistant to microbial decay, certain fungi are able to degrade lignin to carbon dioxide. Many such fungi belong to the Class Basidiomycotina. Of these, P. chrysosporium has been shown to mediate extensive degradation of lignin. In addition to its ability to degrade environmentally persistent naturally occurring compounds it has been shown that P. chrysosporium is also able to mediate extensive degradation of some of the most environmentally persistent synthetic organic pollutants known. Chlorinated compounds degraded to carbon dioxide by this fungus include polychlorinated biphenyls, chlorinated dioxins, the wood preservative pentachlorophenol and the insecticides DDT and chlordane. Chlorinated anilines which are metabolites of a number of pesticides are also degraded to carbon dioxide by this fungus. Many polycyclic aromatic hydrocarbons, including benzo[a]pyrene and phenanthrene are degraded by P. chrysosporium as are the explosives 2,4,6-trinitrotoluene and RDX. A number of studies have shown that the ability of P. chrysosporium to degrade such a wide variety of organic pollutants is due, at least in part, to its lignin degrading system which is expressed when fungal cultures become limited in nutrient nitrogen and/or carbon.

Selected Publications

Bumpus, J.A (2012) A Theoretical Investigation of the Ring Strain Energy, Destabilization Energy, and Heat of Formation of CL-20. Advances in Physical Chemistry, Vol. 2012 Article ID 175146, 7 pages.

Seffernick J.L,  A.G. Dodge, M.J Sadowsky, J.A. Bumpus L.P. Wackett (2010) Bacterial Ammeline Metabolism By Guanine Deaminase. J. Bacteriol  192:1106-1112.

Bumpus, J.A. and P. Willoughby (2008) On the Heat of Formation of Nitromethane. Journal of Physical Organic Chemistry 21:747-757.

Bumpus, J.A., M. Trax, A.  Reisdorph, C. Boyd, D. Gilbert, S. Techau and R.M. Ventullo (2007) Cytochrome c from Phanerochaete chrysosporium: Its Amino Acid Sequence and Analysis of Gene Structural Elements. In Silico Biology,  8, 0001. (http://www.bioinfo.de/isb/2007/08/0001/)

Bumpus, J.A., Lewis, A., Stotts, C. and C.J. Cramer, (2007) Characterization of high explosives and other energetic compounds by computational chemistry and molecular modeling. J. Chem. Ed. 84: 329-332.

Lewis, A.L., J.A. Bumpus, D.G. Truhlar and C.J. Cramer (2004) Molecular Modeling of Environmentally Relevant Processes: Reduction Potentials. J. Chem. Ed. 81:596-604.

Bumpus, J.A. (2003) Biodegradation of Azo Dyes by Fungi. In: Handbook of Fungal Biotechnology, 2nd edition. (Eds. D.K. Arora, P.D. Bridge, D. Bhatnagar and D. Bigelow), Marcel Dekker, Inc., New York, N.Y. (In Press).

Chang H.C. and J.A. Bumpus (2001) Inhibition of Lignin Peroxidase-Mediated Oxidation Activity by Ethylenediamine Tetraacetic acid and N-N-N'-N'-tetramethylenediamine. Proc Natl Sci Counc Repub China B (China ( Republic:1949-)), 25(1):26-33

Chang H. C. and J.A.  Bumpus (2001) Iodide Oxidation and Iodine Reduction Mediated by Horseradish Peroxidase in the Presence of Ethylenediaminetetraacetic Acid (EDTA): The Superoxide Effect.   Proc Natl Sci Counc Repub China B (China ( Republic:1949-)), 25(2):82-9

L.A. Beltz, D.R. Neira, C.A. Axtell, S. Iverson, Wm Deaton,   T.J. Waldschmidt, J.A. Bumpus and  C.G. Johnston (2001) Immunotoxicity of Explosives-Contaminated Soil Before and After Bioremediation. Archives of Environmental Contamination Arch. Environ. Contam. Toxicol. 40:311-317.

Bumpus, J.A. J. J.  Kim and S.R. Coon (2000) Introducing Light Scattering Technology into the Undergraduate Curriculum. J. Chem. Ed. 77:1396-1400.

Axtell, C.A. C.G. Johnston and  J.A. Bumpus (2000) Bioremediation of Soil Contaminated With Explosives at the Naval Weapons Station Yorktown. Soil and  Sediment Contamination 9(6):537-548.

Department of Chemistry and Biochemistry
University of Northern Iowa
1227 West 27th Street
Cedar Falls, IA 50614-0423
Phone: 319-273-2437
FAX: 319-273-7127
E-mail: barbara.reid@uni.edu
Web: http://www.chem.uni.edu

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